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Ferreira, Claudia Gissi Da Rocha;

Ribeiro, André Richter;

Finck, Christiane Madrid;

Percicote, Ana Paula;

Matias, Jorge Eduardo Fouto;

ABSTRACT Purpose: Body weight, blood glucose, and hypothalamic ghrelin receptors were monitored in an animal model of obesity after being treated with sleeve gastrectomy. Methods: Forty-two adult male Wistar rats were randomized into two groups: a non-obese group (standard chow); and an obese group, in which obesity was induced by feeding liquid enteral formula Ensure Plus. Each group was divided according to the surgery performed (sham operation or sleeve gastrectomy) and the time of sacrifice after surgery (14 or 28 days). Body weight and capillary blood glucose were monitored throughout the pre- and postoperative periods. Microscopic sections of the parietal cortex, thalamus, hypothalamus, and hippocampus were treated by immunohistochemical reaction with polyclonal anti-ghrelin receptor antibody. Positivity was determined by identifying labeled nuclei and cytoplasm in the brain cells. Results: Sleeve gastrectomy induced effective weight loss (p < 0.001) and reduction in the hypothalamic ghrelin receptor expression (p = 0.04). Weight loss was not directly influenced by the receptor expression. There was no significant impact on capillary glycemia. Conclusion: Sleeve gastrectomy alters GHSR1a receptors, decreasing their expression and body weight. However, weight loss is not directly related to the GHSR1a expression status.

Tannuri, Ana Cristina Aoun;

Kitahara, Victor Kenji Utino;

Marum, Alexandre Amatruda;

Mola, Bruno;

Souza, Daniel Felipe Marcelino;

Rodrigues, Lucas Menon;

Paes, Vitor Ribeiro;

Leite, Katia Ramos Moreira;

Serafini, Suellen;

Gonçalves, Josiane De Oliveira;

Tannuri, Uenis;

ABSTRACT Purpose: To verify the differences in the reversibility of renal function and damage in rats of different ages with unilateral ureteral obstruction (UUO). Methods: Sixty rats were divided into three different groups, newborns, young, and adults. Twenty-one were sham operated. After UUO, the animals were subdivided into four groups: obstruction (two weeks and sacrifice O2); UUO two weeks, obstruction release for two weeks and sacrifice (R2); UUO for four weeks and sacrifice (O4); and UUO for four weeks, obstruction release for two weeks and sacrifice (R4). Urine was collected for creatinine, urea, total protein, and albumin dosages, and kidneys for morphological studies. Results: Younger rats presented more extensive destruction of renal parenchyma, with some remaining normal tissue healthier in comparison to older rats. The protein excretion by older rats was not different between the groups obstructed for different periods, albuminuria was progressively higher in rats obstructed for longer periods, and after relief of obstruction, there was no difference between newborn rats obstructed for different periods. The younger pyelostomized rats presented higher albumin excretion. Creatinine excretion was worsened in rats after obstruction. Conclusion: Shorter obstruction periods lead to better prognosis, and younger rats showed better recovery after relief of obstruction than older ones.

Zhu, Yan;

Zhang, Yu;

Liu, Feiyan;

Xiao, Jiao;

ABSTRACT Purpose: To examine the protective effect of alpinumisoflavone against streptozotocin (STZ)-induced gestational diabetes mellitus (GDM) in female rats and explored the underlying mechanisms. Methods: Female rats were used in this study, and intraperitoneal administration of STZ (55 mg/kg) was used to induce diabetes. Body weight, blood glucose level, fetuses, placental weight, and placental index were estimated. Oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) were performed. The levels of resistin, glycated hemoglobin (HbA1c), hepatic glycogen, free fatty acid (FFA), adiponectin, serum C-peptide, leptin, visfatin, intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), lipids, oxidative stress, inflammatory cytokines, and other parameters were estimated. mRNA expression was estimated in the pancreatic tissue. Results: Alpinumisoflavone treatment significantly (p < 0.001) enhanced body weight and fetuses and decreased placental weight and placental index. Alpinumisoflavone treatment significantly (p < 0.001) decreased blood glucose levels (BGL) and improved plasma insulin levels. Alpinumisoflavone treatment significantly (p < 0.001) suppressed the glucose and insulin levels in the OGTT and ITT. Alpinumisoflavone treatment significantly (p < 0.001) altered the levels of resistin, HbA1c, hepatic glycogen, FFA, Adiponectin, serum C-peptide, leptin, visfatin, ICAM-1, and VCAM-1; lipid parameters; oxidative stress parameters; inflammatory cytokines and inflammatory parameters, viz., cyclooxygenase-2 (COX-2), and nuclear kappa B factor (NF-κB). Alpinumisoflavone treatment significantly (p < 0.001) altered the mRNA expression levels of Toll-like receptor 4, nuclear kappa B factor65 (NF-κB65), NOD-, LRR-, pyrin domain-containing protein 3 (NLRP3), MyD88, SREBP-1, stearoyl-CoA desaturase-1 (SCD1), FAS, and ACC. Conclusion: Alpinumisoflavone has a protective effect against STZ-induced GDM via alteration of the TLR4/MyD88/NF-κB signaling pathway.

Barros, Rui Sergio Monteiro De;

Santos, Deivid Ramos Dos;

Yamaki, Vitor Nagai;

Teixeira, Renan Kleber Costa;

Valente, André Lopes;

Silveira, Tiago Santos;

Dias, Carmen Gilda Barroso Tavares;

ABSTRACT Purpose: To evaluate the regenerative efficacy of an experimental biodegradable nerve conduit composed of polycaprolactone and açaí-derived polyurethane, used for peripheral nerve repair, in comparison with different reconstructive techniques. Methods: Wistar rats (n = 48) were allocated into six groups (n = 8): normality (NG), denervated (DG), burial (BG), nerve autograft (NAG), açaí-based neurotube (ANG), and vein autograft (VAG). Sciatic functional index, electrophysiological parameters, and histomorphometry were assessed after 12 weeks. Results: NAG and ANG showed significant functional recovery, with ANG being the only group to demonstrate progressive improvement (p = 0.009). Electrophysiological analysis revealed higher amplitude and lower latency in NAG, followed by ANG and VAG. Histomorphometric analysis showed increased axonal density in ANG and NAG compared to non-reconstructed groups (p = 0.004 and p = 0.007, respectively). Conclusion: The polycaprolactone/açaí-based conduit demonstrated regenerative performance comparable to autologous nerve grafts.

Falco Neto, Wilson;

Domingos, Pedro Henrique;

Okida, Gabriel Sanchez;

Pissolito, João Felipe;

Sanches, Pedro Henrique Soubhia;

Azevedo, Lucas Ribeiro De;

Mourão Junior, Marcelo Oliveira;

Sanches, José Antônio;

Gragnani, Alfredo;

ABSTRACT Purpose: To analyze the geographical distribution of patients treated at a burn treatment unit (BTU) in Catanduva, São Paulo, Brazil, and examine the relationship between geographic and clinical variables. Methods: This is a cross-sectional study that analyzed patients hospitalized for burns between January 2018 and May 2022. Data were obtained from medical records and included patients’ residence city, age, and percentage of total body surface area burned (%TBSA). The data were processed using QGIS and R, and travel distances and times were calculated. Statistical analyses included bivariate and correlation tests. Results: The total of 1,164 patients were analyzed. Most of them resided outside Catanduva, totaling 277 cities. The average distance was 179.37 km, and the average travel time was 140.94 minutes. Patients from Catanduva had an average age of 35.55 years old, and the average %TBSA was 12.15. Patients from outside Catanduva were significantly younger and had a higher %TBSA than the local patients. A weak but significant negative correlation was found between patient age and distance to the BTU (ρ = -0.14, p < 0.05), while %TBSA showed a weak positive correlation with travel distance (ρ = 0.21, p < 0.05). No significant differences were observed between the pre- and pandemic periods. Conclusion: This study highlights regional differences in specialized burn care access and may inform policy aimed at reducing care disparities.

Abate, Juan Cruz;

Marinoff, Ivana Ivanoff;

Arnal, Nathalie;

Machuca, Mariana;

Papa-Gobbi, Rodrigo;

Vecchio, Leandro;

Rumbo, Martín;

Stringa, Pablo;

Lausada, Natalia Raquel;

ABSTRACT Purpose: To mitigate ischemia-reperfusion injury (IRI) triggered in solid organ transplant procedures, we aimed to evaluate the effects of multi-organ abdominal ischemic preconditioning (MAIP) in the context of renal IRI. Methods: An experimental kidney transplant model was conducted. Rats were divided into three groups: an intervention free basal group from which physiological data was collected; a control group (CT), which consisted of transplanted animals without MAIP; and a treated group, in which a MAIP protocol was implemented in the donor during the procurement of the left kidney, monitoring the recipient for 24 hours. Results: Urea, creatinine, and lactate dehydrogenase, as well as histopathological analysis (Banff: CT 1,66 ± 0,57 vs. basal 0, and MAIP 1), showed a clear trend in favor of MAIP group. Similar results were observed for tumor necrosis factor-α, interleukin-6 and CXCL10, as well as indicators of oxidative stress, with statistically significant levels for CXCL10 [0,295 ± 0,0074 arbitrary units (AU) CT and 0,0057 ± 0,0065 AU MAIP] and TBARS (2,93 ± 0,08 nmol/μg CT; and 2,49 ± 0,23 nmol/μg MAIP; p 0.05). Conclusion: The findings indicated that the MAIP exerts a protective influence on the transplanted kidneys, functioning as an IRI-protective strategy and enhancing the parameters associated with renal graft functionality.

Souza, Andrea Pelicia Roso De;

Palhares, Raquel;

Baltieri, Letícia;

Mussi, Ricardo Kalaf;

Antunes, Edson;

Mello, Glaucia Coelho De;

ABSTRACT Purpose: To establish a profile of the inflammatory response in the preoperative and postoperative period of pulmonary resection of patients without postoperative complications, in order to trace the inflammatory profile of lung resection surgery. Methods: Six collections of arterial and venous blood were performed for data analysis, one sample in the preoperative, immediate postoperative, 4, 8, 24, and 48 hours after surgery. Twenty-seven patients with a median age of 63 years old, ranging from 29 to 80 years old, were included. Results: The leukocyte count showed a significant increase in the times: immediate postoperative and 4 hours after surgery, in relation to the preoperative period. Concomitantly, there was an increase in lactate, heart rate, interleukin (IL)-6 and IL-8 after 4 hours of surgery. The platelet count showed a significant decrease in 48 h, associated with an increase in IL-1β and tumor necrosis factor-α. A significant increase in IL-10 was observed in the immediate postoperative. Conclusion: The study may contribute to the search for more specific and adequate alternatives for controlling the inflammatory response. In this way, the intervention would be specific to that cytokine that causes the greatest harm to the patient, as well as to the moment of the intervention.

Karaaslanlı, Abdulmutalip;

Tuncer, Mehmet Cudi;

Aşır, Fırat;

Korak, Tuğcan;

ABSTRACT Purpose: We aimed to investigate the role of gallic acid treatment on spinal cord tissues after spinal cord injury (SCI) and its relationship with endoplasmic reticulum (ER) stress by histochemical, immunohistochemical, and in-silico techniques. Methods: Thirty female Wistar albino rats were divided into three groups: sham, SCI, and SCI+gallic acid. SCI was induced by dropping a 15-g weight onto the exposed T10-T11 spinal cord segment. The SCI+gallic acid group received 25 mg/kg of gallic acid intraperitoneally daily for one week. Histopathological, immunohistochemical, and silico analyses were performed. Results: Histological analysis revealed improved neural cell survival and tissue integrity in the SCI+gallic acid group compared to the SCI group. Caspase-12 expression was significantly increased in the SCI group, indicating elevated ER stress and apoptosis. Gallic acid treatment resulted in a marked reduction in caspase-12 expression in neurons, neuroglia, and endothelial cells, suggesting decreased ER stress. Conclusion: Gallic acid exhibits significant neuroprotective effects against ER stress and cellular damage in a rat model of SCI. The in-silico analysis revealed apoptotic and immune-related pathways in which gallic acid showed neuroprotective effects by regulating caspase-12. These results suggest that gallic acid may be a promising therapeutic agent for mitigating secondary damage post-SCI.

Oliveira, Evandro Luis Da Cunha;

Vicente, Yvone Avalone De Moraes Villela De Andrade;

ABSTRACT Purpose: The aims of this work were to induce anorectal anomaly in rat fetuses via the planned administration of ethylenethiourea (ETU), and to study fetuses exhibiting anorectal malformation, as well as apparently normal fetuses submitted to the effect of ETU. Methods: Time-mated pregnant Wistar rats were randomly divided into control and experimental groups. On gestational day 10, the experimental group received 10% ETU (130 mg/kg) by gavage, whereas the control rats received vehicle only. The embryos were harvested by cesarean section on gestational day 21. The fetuses exposed to ETU were divided into two groups: affected (without any clear anorectal alterations); and the malformed (with anorectal anomaly). The neuromotor plates were identified by immunohistochemistry with acetylcholinesterase, and alterations in the sacral region were evaluated by histological and morphometric studies. Results: We used 43 control fetuses, 82 affected fetuses, and 118 malformed fetuses in this study. The most frequent associated macroscopic anomalies were spina bifida (55 fetuses), encephalocele (20), and alterations in the lower limbs (5). The sacroiliac was malformed in 45% of the affected fetuses and in 53.2 % of the malformed fetuses. Conclusion: ETU leads to a reduced number of motor neurons in the pelvic musculature of both the malformed and the affected rats. The enteric neurons are altered in the malformed fetuses, but not in the affected ones. Both the affected and malformed rats exhibit sacral alterations that do not interfere with neurons.