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Almeida, Vinícius Lima De;

Ferraz, Danilo Cassiano;

Cabral, Giovanna Miranda;

Gobbi, Arthur Henrique;

Vieira, Walbert De Andrade;

Lima, Lívia Bonjardim;

Lima, Rafael Rodrigues;

Rode, Sigmar De Mello;

Paranhos, Luiz Renato;

ABSTRACT Purpose: To conduct a bibliometric analysis of the 100 most-cited articles on the use of complementary therapies for controlling inflammatory signs, symptoms, and complications after lower third molar extraction. Methods: An electronic search was performed on the Web of Science Core Collection database. A graphical bibliometric network was created in Power BI software (Microsoft, Redmond, WA, United States of America). Spearman’s correlation test was used to assess the correlation among the number of citations, journal impact factor, and the year of publication. Results: The selected articles received 4,199 citations, covering the years 1983 to 2023. Most publications occurred in 2016. Article citations averaged 41.99. The most frequent Keywords Plus terms, considering more than five occurrences, were “removal,” “pain,” “surgery,” and “trismus.” The main keywords defined by the authors, considering more than five occurrences, were “pain,” “swelling,” and “trismus.” The Asian and European continents presented the highest number of publications and citations. Randomized clinical trial was the most prevalent study type. Regarding authors’ affiliations, the University of Turin stood out in the number of publications. Conclusion: The article with the highest number of citations was published in Serbia. Turkey and Italy accounted for the highest number of publications and citation density. Although most included studies were randomized clinical trials, improving comparison and decision-making requires methodological standardization.

Arslan, Ayla;

Keleş, Dilek Vural;

Parlak, Seçil Nazife;

Yakut, Seda;

Gündoğdu, Habibe;

Arslantürk, İrem;

Uzun, Gökçe Bağcı;

Karagöz, Berna Öztürk;

ABSTRACT Purpose: To evaluate the protective effects of nicotinamide mononucleotide (NMN), a NAD+ precursor, on kidney tissue in rats with sepsis induced by cecal ligation and puncture model. Methods: In this study, 28 female Sprague Dawley rats were randomly divided into four equal groups: control, sham, sepsis, and sepsis+NMN (500 mg/kg, i.p.). Kidney tissues taken 24 hours after the sepsis model were histopathologically evaluated under light microscopy for tubular damage, glomerular congestion, and inflammatory infiltration parameters. Results: Histopathological examination revealed severe tubular injury, marked glomerular congestion, and dense inflammatory cell infiltration in the kidney tissues of the sepsis group. In the group treated with NMN, a significant reduction in these pathological findings was observed, while the glomerular structure and tubular architecture were preserved. Semi-quantitative histopathological scoring confirmed significantly increased renal injury in the sepsis group and significant attenuation of tissue damage following NMN treatment. Conclusion: The data obtained showed that NMN may play an important cytoprotective role in sepsis-induced acute kidney injury by preserving tissue architecture. More comprehensive molecular and biochemical studies are needed to fully elucidate the therapeutic potential and mechanisms of action of NMN.

Anadolulu, Ali İhsan;

Aydöner, Sinem;

Pirim, Ahmet;

Ozkanli, Seyma;

Durakbaşa, Çiğdem Ulukaya;

ABSTRACT Purpose: To investigate the early and late effects of hydroxychloroquine (HCQ) on inflammation, fibrosis, and apoptosis in an experimental rat model of pulmonary contusion. Methods: Thirty-five male Wistar albino rats were randomly allocated into five groups (n = 7): control, pulmonary contusion evaluated on day 3 or day 10 (PC-3 and PC-10), and pulmonary contusion treated with HCQ at 100 mg/kg/day by gavage and evaluated on day 3 or day 10 (PCHCQ-3 and PCHCQ-10). Lung injury was assessed histopathologically by alveolar edema, congestion, leukocyte infiltration, and fibrosis scores and immunohistochemically by caspase-3, inducible nitric oxide synthase (iNOS), and endothelial nitric oxide synthase (eNOS) expression. Results: No alveolar edema was observed. Congestion and leukocyte infiltration were significantly greater in all contusion groups than in the control group. Fibrotic changes were detected on day 10 in both treated and untreated contusion groups. Caspase-3 and iNOS expression were significantly higher in the contusion groups than in the control group. HCQ did not significantly reduce leukocyte infiltration, fibrosis, apoptosis, or iNOS expression compared with the corresponding untreated groups. Conclusion: HCQ administered at 100 mg/kg/day for three or 10 days did not demonstrate significant anti-inflammatory, anti-apoptotic, or antifibrotic effects in this rat model of pulmonary contusion.

Pacheco, Leticia Stefani;

Possiedi, Rafael Dib;

Scalea, Thomas Michael;

Ribeiro Junior, Marcelo Augusto Fontenelle;

ABSTRACT Purpose: Trauma is a major global cause of death, with uncontrolled hemorrhage as its leading preventable cause. Damage control resuscitation (DCR) is a cornerstone strategy for hemorrhagic shock, yet implementation varies by resource availability. This study compared DCR practices between high-income countries (HICs) and low- and middle-income countries (LMICs), predominantly represented by institutions from Latin America, particularly Brazil. Methods: A cross-sectional survey of trauma care providers was conducted between 2024 and 2025, assessing DCR practices, resuscitation strategies, and adjunctive therapies. Data from a Brazilian national survey were incorporated into the LMIC cohort. Countries were classified using World Bank 2024 income criteria, and comparisons were analyzed using hierarchical logistic regression with adjustment for multiple comparisons. Results: Massive transfusion protocol availability result was significantly lower in LMICs than in HICs (47.4 vs. 89.1%; OR = 0.11, 95%CI 0.02–0.62; p(BH) < 0.001). LMICs also had reduced availability of fresh frozen plasma (OR = 0.04; p(BH) = 0.035), balanced crystalloids (OR = 0.21; p(BH) < 0.001), viscoelastic coagulation monitoring (TEG/ROTEM; OR = 0.04; p(BH) 0.001), prothrombin complex concentrate (OR = 0.01; p(BH) < 0.001), fibrinogen concentrate (OR = 0.10; p(BH) < 0.001), rapid infusers (OR = 0.04; p(BH) < 0.001), invasive temperature monitoring (OR = 0.02; p(BH) < 0.001), calcium replacement (OR = 0.17; p(BH) = 0.002), and active rewarming systems (OR = 0.21; p(BH) = 0.035). Whole blood use was lower in LMICs (5.8 vs. 17.4%) but did not reach statistical significance in the primary model (OR = 0.55, 95%CI 0.12–2.45). Tranexamic acid availability was nearly universal and did not differ between settings (96.1 vs. 97.8%). Conclusion: Although DCR principles are widely recognized, substantial disparities persist in access to critical resuscitation resources across income settings.

Fortes, Marcos Fraga;

Fortes, Gabriela Noronha;

Fortes, Marcos Noronha;

Gomes, Fernanda Christoffel;

Seelaendar, Marilia;

Pinho, Ariene Murari Soares De;

Jesus, Joyce De Cassia Rosa De;

Otoch, José Pinhata;

ABSTRACT Purpose: Cancer-associated cachexia (CC) is characterized by involuntary weight loss and poor prognosis. Central nervous system (CNS) inflammation has been linked to CC symptoms. This study evaluated inflammatory mediators in the cerebrospinal fluid (CSF) of patients with CC. Methods: Volunteers were recruited in Santarém, PA, Brazil, and divided into three groups: patients undergoing herniorrhaphy or cholecystectomy (n = 43)—control; cancer without cachexia (CWC, n = 13); and CC (n = 49). Blood and CSF were collected before anesthetic injection. Results: CSF vascular endothelial growth factor (VEGF) and monocyte chemoattractant protein-1 (MCP-1) levels were reduced in CC (p < 0.0001; p 0.00017) and CWC (p = 0.0269; p = 0.0421) compared to control. CSF IP-10 was lower in CC (p = 0.0048). In CWC, CSF MCP-1 correlated with serum C reactive protein (rs = 0.78, p = 0.0098), leukocytes (rs = 0.72, p = 0.0235), lymphocytes (rs = -0.67, p = 0.0390) and monocytes (rs = -0.82, p = 0.0058). In CC, CSF interleukin (IL)-8 correlated with leukocytes (rs = 0.48, p = 0.0032) and MCP-1 with neutrophils (rs = 0.36, p = 0.0265) and eosinophils (rs = -0.37, p = 0.0218). CC patients showed lower quality of life than CWC (functional scale p = 0.0087; symptoms scale p = 0.0386). Conclusion: Cancer was related to changes in CSF cytokine levels, indicating that both tumor presence and cachexia modulate central inflammatory signaling. Correlations between CSF chemokines and blood immune cells suggest systemic interaction between peripheral immunity and the CNS.

Sousa, Willane Bandeira De;

Nogueira Neto, João;

Araújo, Kauã Manuel Costa;

Gomes, Lyvia Maria Rodrigues De Sousa;

Cartagenes, Maria Do Socorro De Sousa;

Garcia, João Batista Santos;

ABSTRACT Purpose: To map the experimental models of induced and spontaneous uterine leiomyomas (UL) in animals, describing induction techniques, evaluation methods, advantages, limitations, and research gaps. Methods: We conducted a systematic review of studies published between 2004 and 2024 with in-vivo animal experiments reporting UL induction or spontaneous occurrence. Data were extracted on animal species, sample size, induction methods, confirmation techniques, and reported advantages or disadvantages. Results: A total of 56 studies were included. Rats and mice were the most frequently used species. Induction methods comprised hormonal administration, genetic predisposition, chemical exposure (monosodium glutamate), ischemic injury, external stimulation, cell transplantation, xenotransplantation, and spontaneous development. A critical finding that emerged was that 60.7% of the studies failed to report confirmation of UL induction. Among the 22 studies that reported confirmation, caliper measurement was the most frequently used technique. This widespread lack of methodological rigor significantly undermines the credibility and reproducibility of many published models. Conclusion: This scoping review highlights not only the heterogeneity of UL animal models and the lack of standardized induction and evaluation protocols, but also identifies a critical gap in induction validation reporting that compromises the foundation of preclinical research in this field.

Andrade, Bruno Tavares De;

Lopes, Carlo Átila Holanda;

Lima, Yury Tavares De;

Pincanço, Victor Rocha;

Bisio, Natan Rolim De Assunção;

Sa, Henrique Luis Do Carmo E;

Castro Neto, Josué Viana De;

ABSTRACT Purpose: This study described the development and preliminary evaluation of a simulator that combines a three-dimensional-printed vascular model with a camera-assisted system for training basic endovascular skills. Methods: A hollow mannequin torso housed a transparent, three-dimensional-printed aorta. Guidewire and catheter manipulation were visualized in real time using a manually controlled camera. Fifteen medical students (novices) and five vascular surgeons (experts) performed simulated tasks in two sessions. Experts also assessed realism and educational value. Results: Novices demonstrated shorter completion times across all exercises in session 2, particularly in exercise 4, with mean time decreasing from 229.3 ± 134.8 to 83.5 ± 31.1 seconds, a mean difference of 145.8 seconds (p = 0.001). Experts showed no significant improvement in most tasks, although exercise 3 improved from 115.4 ± 29.7 to 91.6 ± 21.5 seconds, a mean difference of 23.8 seconds (p = 0.012). Performance differed between groups (construct validity). Surgeons provided favorable ratings for the realism and usefulness (face and content validity). Conclusion: This preliminary device appears feasible and may facilitate early skill acquisition in low-resource educational settings without radiation exposure.

Silva, Amanda Silveira Da;

Barbosa, Stephannie Jamyla De Araújo;

Ribeiro, Susana Barbosa;

Carvalho, Lucas Vinicius Souza De;

Silva, Rafaela Torres Dantas Da;

Guerra, Gerlane Coelho Bernardo;

Araújo Júnior, Raimundo Fernandes De;

Silva, Nizyara Costa Da;

Andrade, Raphael Victor Silva;

Paiva, Gabrielle Marques De;

Langassner, Silvana Maria Zucolotto;

Dantas-Medeiros, Renato;

Fé, Rafaela Alcindo Silva De Sousa;

Lima, Maria Laura De Souza;

Araújo, Aurigena Antunes De;

ABSTRACT Purpose: This study aimed to evaluate the effect of oral administration of a hydroethanolic extract of Commiphora leptophloeos leaves (CLF) on the progression of 5-fluorouracil (5-FU)-induced intestinal mucositis. Methods: Female Swiss mice were orally administered CLF at doses of 10, 100, or 300 mg/kg, or saline (negative control, NC), starting 30 minutes before intraperitoneal injection of 5-FU. A positive control (PC) group received 5-FU without CLF treatment. Treatment continued until euthanasia, three days after 5-FU injection. Body weight was monitored throughout the experiment. On day 4, small intestine tissue samples were collected for histopathological analysis. Duodenal tissue was also assessed for Muc-2 immunostaining. Results: In the CLF 10, 100 and 300 mg/kg groups, the duodenum histopathological score was 1 (1–1.5) p < 0.001, 1 (1–1.5) p < 0.01; 1 (1–1.25) p < 0.01, while the PC group score was 2 (2–2.75). Immunostaining analysis for Muc-2 revealed a score of 3 (3–3) in the CLF 10 group, compared to score 1.2 (0.8–1.6) in the PC group (p < 0.001), indicating that CLF 10 increased Muc-2 expression. Conclusion: The oral administration of a hydroethanolic extract of CLF protected the duodenal segment of Swiss mice from 5-FU-induced intestinal mucositis, likely by increasing mucin production.

Aranha Junior, Ayrton Alves;

Matias, Jorge Eduardo Fouto;

Veronez, Djanira Aparecida Da Luz;

Noronha, Lucia De;

Repka, João Carlos;

Aranha, Rafaella Toledo;

Campos, Antônio Carlos Ligocki;

ABSTRACT Purpose: To evaluate the effects of maternal arginine-enriched diet during lactation, following gestational undernutrition, on offspring growth and wound healing. Methods: Pregnant Wistar rats were divided into two groups during gestation: control (C; ad libitum diet) and undernourished (Und; 60% of control diet). After delivery, the undernourished mothers were reassigned to two subgroups: ad libitum + Impact® (Arg) and ad libitum + Nutren® (Hh). Evaluated parameters included offspring anthropometric measurements, serum albumin, tensile strength, and collagen morphometry at the surgical wound. Statistical analyses included analysis of variance (ANOVA) and Tukey’s, Student’s t-test, and Fisher’s test. Results: Maternal immunonutrition during lactation promoted recovery of offspring weight (C = 29.21 ± 2.86 g; Arg = 36.08 ± 5.38 g; p = 0.0009) and body length (C = 9.87 ± 0.32 cm; Arg = 10.91 ± 0.73 cm; p = 0.0008), shifting values from significantly lower at birth to higher than controls by weaning. Offspring healing parameters showed no significant differences compared with controls. Conclusion: An arginine-enriched diet provided to lactating rats malnourished during gestation significantly improved offspring anthropometric growth and supported wound healing following surgery at weaning.